protein x Freund's adjuvant

Pete immuno-affinity at rocketmail.com
Fri Feb 13 07:11:16 EST 1998


ita at DNA.CBIOT.UFRGS.BR (ITABAJARA DA SILVA VAZ JUNIOR) wrote:

>
>I need to remove the Freund's Adjuvant from a protein extract.
>Is it possible?
>thanks 

I suppose, if you centrifuge it you will get a minearal oil upper
phase, a lower aqueous phase containing your (soluble) protein
extract, and a pellet containing the mycobacteria (if it was complete)
and any other insoluble protein etc.

Pete



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