How to make serum for tissue culture?

L.A. Pike-Nobile ez063669 at mailbox.ucdavis.edu
Mon Feb 16 21:43:08 EST 1998


John,

Perhaps I'm missing something here, but if you heat-inactivate your serum
(56oC, 1 hour) doesn't that cook the remaining B2m and make it unavailable
for complexing with MHC I? Particularly since you have an antibody which
recognizes an epitope spanning the two molecules, I'd think heating (and
pehaps addition of a little 2ME) would eliminate your problem by
eliminating the ability of B2m to complex with the a3 domain of MHC I. 

Or am I missing something fundamental about small globular proteins? 

Regards,
Larry





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