How to make serum for tissue culture?

[Ken Theil]

Hi.

One possibility is to use a series of filters of different porosities, the 
first being one with a large pore diameter (equal to or even greater than 0.8 
micron) coupled with a prefilter.  These filters could be premoistened by 
passing a small volume of cell culture medium through them (which is then 
discarded), and then pass the serum through it.  Then take the 
resulting filtrate and pass it through smaller filter (0.45 or 0.22 micron 
without a prefilter).  Good luck.

KW Theil
Wooster, Ohio