Dear Kim Tae Yoon,
for the culture of many different cell lines the classical media like RPMI
or D`MEM have to be supplemented for optimal growth or survival of the
employed cells et all. Because some of these reagents (pyruvate, non
essential aminoacids etc.) have a short lifetime in an aequous solution they
are supplemented directly before the preprepared medium (which is usually
sterile-filtered and stored in a refrigerator) is used e.g. to split the
cell population you are growing.
Best wishes
Dr. W. Splettstoesser, M.D., Munich - Germany
Kim Tae Yoon schrieb in Nachricht <34F288B9.37CC15BB at cmc.cuk.ac.kr>...
>I recently start T lymphocyte culture. I used comercial RPMI1640 power
>media.
>>In many reference additional suppliment including glutamin, 2-ME,
>pyruvate etc.
>>Why these suppliment was needed?
>>Please let me know.
>>Thanks and may God bless you and me.
>>JP
>
