Is spleen cell could be long-term cultured without interleukin?

[J.S Good]

In article <6d0gk9$8ki6 at hkusud.hku.hk>, "Adrian Wu"
<adrianwu at hkucc.hku.hk> wrote:

> First of all, make sure you put 2-ME in the medium. 

What does it do?