I am interested in analyzing cell cycle of a breast cancer cell line by using
Ki-67.
I have noticed that in using DAB staining that the nucleoli are the first to
"light up." When counting the cells (using traditional light microscopy),
there are about 80 of the positively staining population which have
prominently stained nucleoli but with a counterstain background in the rest of
the nucleus (hematoxylin).
We have conflicting points of view among our labs: is this specific or
nonspecific staining?
Since 70% of the entire cell population does not show any nucleolar staining,
my guess is that it is specific.
Would anyone know of any references in published literature that addresses
this question?
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