Help with NGF immunoassay (ELISA)
Robert W. Andersohn
gigan at grove.ufl.edu
Wed Jan 21 20:20:11 EST 1998
I am a student researcher from the Univ. of Florida, and I have been
trying to use an "indirect sandwich" ELISA to measure NGF in postnatal
rat brain. I have run in to some complications. Our values for NGF
levels are well over the consistent values published over recent years.
I would like to know if someone has had any problems setting up an NGF
ELISA in their lab. I would also like to know if there are any
suggestions as to why we cannot seem to get reasonable data (ANYTHING).
We have spent a lot of time trying to elucidate factors which could be
skewing our results and have searched the literature thoroughly. We
have been "measuring" NGF in 4,10, and 21 day-old H-Tx rats from
striatum, hippocampus, and posterior cortex using the "NGFemax" system
from Promega Corp.
Please respond directly by email (mailto: coriakin at grove.ufl.edu) or
post a message here. Any help would be greatly appreciated.
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