IgY Extraction, pleas HELP!?

silvis_r at hotmail.com silvis_r at hotmail.com
Thu Jun 4 07:34:57 EST 1998


Hello, i need some help with IgY extraction from egg yolk.

I got a tip from someone to put the egg yolk overnight at 4 degr Centigrade
in Phosphate bufferd salt (PBS). the following morning i added 3,5% of PEG
6000 to remove any unwanted lipoproteens and other proteens. After
centifugion at 5000 G at 30 min i got the wanted sediment. I than added 12%
PEG 6000 to the supernatant and swong it around at 5000 G for 30 min at
first, i got no result so i have put it back at 12000 G 30 min., still i have
no result.

What went wrong, why doesn't my IgY form a sediment, i have a turbid
suspension!?

Tanx for your help

-----== Posted via Deja News, The Leader in Internet Discussion ==-----
http://www.dejanews.com/   Now offering spam-free web-based newsreading



More information about the Immuno mailing list