Problem about T Cell Purification
CHAN Ella Wai Ching
boella at uxmail.ust.hk
Fri Jun 5 01:19:29 EST 1998
I would like to know how to use percoll for T cell purification from
I have tried to set the percoll gradients using 3ml 1.06g/ml, 4ml 1.07g/ml
then follwed with 1.08g/ml. I loaded 4ml of heparinized blood over the
gradient and centrifuged for 25 minutes at 1200rpm (300 x g), room temp,
no break. However, it was found that the separation wasnt clear and it was
quite hard for me to distingulish each cell bands.
What kind of gradient should I setup for such kind of purification??
Thanks a lot for your attention.
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