help western-blott problem

Hao Xiao haoxiao at
Fri Jun 5 21:00:53 EST 1998

I experience non-specific binding in western-blotting system of mouse tissue

My primary Abs are immunoaffinity purified rabbit Ab against pY, ERK1, P38
(hog1) and p53. All my blots contained nastic bands corresponding to
immunoglobulins.  I thought it is attributed to the cross-reactivities of
anti-rabbit Ig's to mouse Igs. But the bands are intensified through
increasing the conc. of primary Ab but not the secondary. Pleasae help.

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