It is possible to run ELISPOT and proliferation at the same time using the same animals?

mark mark.haynes at
Tue Jun 9 13:46:51 EST 1998

Kefei Hu wrote:
> In order to minimise the cost both in terms of time and money as well as
> to use as few animals as possible
> to evaluate immune response induced by a vaccine
> for example, Is it possible to use the same animals for Ig or cytokine
> ELISPOT and proliferation test at the same time? Here I mean to get good
> results for all of tests involved and I understand that the ideal
> timings for Ig ELISPOT and proliferation are different.

I can't see why not--the issue would be the numbers of cell isolated from 
each individual animal.  but what other issues would prevent it?

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