staining Drosophila S2 cell nuclei

Toby Lieber liebert at rockvax.rockefeller.edu
Wed Jun 17 14:18:40 EST 1998


Hi,

I have been trying to counterstain nuclei of Drosophila S2 cells that have
been fixed in paraformaldehyde. As we don't have a confocal with a UV
laser the stain has to be either red or green. I have tried propidium
iodide, which does not stain at all, and SYTOX Green which does stain but
is very unstable in S2 cell nuclei and does not last over night. Does
anyone have any suggestions?

Thanks,
Toby

PS please cc me.



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