Why CD3 coated plate is used in T cell cloning?
decken at rz.uni-leipzig.de
Tue Mar 3 08:46:11 EST 1998
Kim Tae Yoon wrote:
> In many case CD3 coated 96-well plate was used.
> Please let me know the reason. And where can I
> purchase that product.
You need it for polyclonal, i.e. unspecific stimulation of T-cells. I
don't know any commercial source of coated plates. I just coat them
myself. Dilute about 1 microgram of monoclonal anti-CD3 per ml of the
appropriate cell culture medium. Pipet 100 microliters of the diluted
antibody into each well and incubate over night (or at least for one
hour). Discharge the antibody before adding cells to the wells.
Klaus Decken mailto:decken at rz.uni-leipzig.de
Veterinary Medicine http://www.uni-leipzig.de/~immun
University of Leipzig
Zwickauer Str. 53 Telephone: (+49/0) 341 97 38 343
D-04103 Leipzig Telefax: (+49/0) 341 97 38 349
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