tom wrote:
>> Hi everyone
>> I have been using IMS-PCR technique for detecting bacterial cells in
> seeded faeces. I am using Dynal M280 and M450 beads coated with
> monoclonal antibodies. However, when I use uncoated beads (ie. no Ab), I
> find I am also getting a positive PCR result. Has anyone else using these
> beads come up with similar problems ? I would be most appreciative of any
> suggestions/comments people can give me on this technique. Thanks in
> advance.
>> Tom
>> -----------------------------------------------
> Tom La, PhD Student
Tom what are your specific steps in the protocol? How do you block
blank beads for instance?
markH
