IMS-PCR

mark mark.haynes at mail.tju.edu
Thu Mar 5 18:01:41 EST 1998


tom wrote:
> 
> Hi everyone
> 
> I have been using IMS-PCR technique for detecting bacterial cells in
> seeded faeces.  I am using Dynal M280 and M450 beads coated with
> monoclonal antibodies.  However, when I use uncoated beads (ie. no Ab), I
> find I am also getting a positive PCR result.  Has anyone else using these
> beads come up with similar problems ?  I would be most appreciative of any
> suggestions/comments people can give me on this technique.  Thanks in
> advance.
> 
> Tom
> 
> -----------------------------------------------
> Tom La, PhD Student

Tom what are your specific steps in the protocol?  How do you block 
blank beads for instance?
markH



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