Dendritic cell isolation from mouse lymph node and spleen

Kim Tae Yoon tykimder at CMC.CUK.AC.KR
Mon Mar 9 12:31:11 EST 1998

Hi, there!

I use MACS CD11c microbeads to isolate dendritc cell from mouse spleen.
Except magnetic labelling, I don't use any procedure such as culture for
overnight or CD4-panning to deplete T cells or Fc-rosetting.
I just hope to know whether or not it is possible to isolate DC with
high purity using only magnetic labelling.  Otherwise, should I use
other procedures to isolate DC with high purity?
And if you know another method to isolate DC, let me know it, please!

Good luck to you!!

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