Dendritic cell isolation from mouse lymph node and spleen

Timothy L Wyant twyant at umaryland.edu
Fri Mar 13 17:00:44 EST 1998


Hi Kim,

CD11c is found on ALL monocyte derived cells.  When you do what you are
doing you are selecting more than likely for the macrophages. If your
ultimate goal is to add TNF-alpha/IL-4 or GM-CSF to generate DC you are on
the correct tract. If you believe they are only DC being isolated, try
again.

Good luck

Dr.Tim Wyant




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