islet cells culture of NOD mice

mark mark.haynes at mail.tju.edu
Mon Mar 16 18:52:06 EST 1998


Philippe Halbout wrote:
> 
> I'm working on IDDM of NOD mice and I need any information about B
> cells of Langerhans islets culture. Thank you for
> references, e-mail, articles wich could help me.


Look up Paul Lacy papers from the 80's.  They developed a protocol for 
isolating islets by perfusing pancrease-in situ with enzymes.
good luck its not easy, but maybe the techniques have been improved upon. 
 Do you really need islets could you use transformed cells for your 
studies?



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