intracellular cytokine detection

Timothy L Wyant twyant at umaryland.edu
Tue Mar 17 13:21:18 EST 1998



I forget to place in our procedure that 5 hours before the "harvesting" of
our cells we also add monensin (2.5um) to allow for the accumulation of
the cytokines.  Keep in mind, cytokines like IL-1b do not have a signla
sequence and therefore do not traffic through the golgi and monensin does
not allow for the build up of this cytokine.

Dr. Tim Wyant





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