Platelet contamination

[Greta Burman]

David Verhoeven wrote:
> 
> I isolate PBMCs from blood utilizing a Ficol gradient.  The only problem
> is that I still get comtamination with platelets.  Is there a way to get
> rid of the platelets from my PBMCs?

A way to deplete platelet is low rpm centrifugation (800 rpm) but you
will also loose many PMNC. I rutinelly work with PMNC, doing flow
cytometry, immunohistochemistry and cell culture. I never had problems
because of platelets around.

Corrado

Dept Cell and Mol Biol
Univ Umea, Sweden