Platelet contamination

[Doris Anne Morgan]

An additional step to low-speed spin is to layer very gently 10-15 ml of the
cell suspension over an equal vol of fetal calf serum. Centrifuge 800 rpm,
10 min at 15o. This may help  in decreasing the contaminating platelets.

David Verhoeven wrote:

> I isolate PBMCs from blood utilizing a Ficol gradient.  The only problem
> is that I still get comtamination with platelets.  Is there a way to get
> rid of the platelets from my PBMCs?