immunization specialists

Patricia Werner werner at inw.agrl.ethz.ch
Mon May 4 02:39:58 EST 1998


I am not sure what to do with my immunization. I have a recombinant
protein, containing a 6xhis-tag at the  N-terminus. The protein is 
isolated and purified under denaturing conditions, but I have problems 
removing the his-tag. 

Does anybody have experience in immunizing animals with a protein 
containing such a tag ? I mean, may such a tag be responsible for
a low antibody production against the protein of interest ? Is it 
possible to guess the antigenity of a 6xhis-tag compared to a protein 
of 170 amino acids ?

I am grateful for any suggestion


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