In article <v03020905b1737bcba9deF@[193.52.76.98]>#1/1,
camoin at cochin.inserm.fr (Luc CAMOIN) wrote:
>> Dear netters,
>> I'am going to try lymphnode immunization in order to produce antibodies
> against a synthetic peptide. Can you inform me if I need to link my peptide
> to a protein carrier as KLHf or if the free peptide could be use?
>> Thanks in advance,
>> Luc CAMOIN
Dear Luc,
It is not possible to answer you without knowing which peptide you want to
use. In principal, some peptides are very good in inducing antibodies. I would
first try to inject the peptide alone, but in an adjuvant, like CFA or Alum.
If this does not work, you can try to couple it to BSA or KLH.
Ari
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