combination of promter and polyA sequence

Jun-Ichi Aikawa jaikawa at UCSD.EDU
Tue May 12 17:43:09 EST 1998

Does anyone know or experince what is good and what is bad combination of
promter and polyA sequence for expression?

Commerical availave vector contains SV40 promoter-SV40 polyA, CMV
promoter-bGH(bovine Growth Hormone) polyA, etc. I'm planning to construct
plasmids one of which contains SRalpha promoter-bGH polyA and another
EF1(Elongation Factor-1) promoter-bGH polyA. I hope these combinations
would work well, but does anyone have any suggestions and comments? Please
let me know.


Jun-ichi Aikawa, Ph.D.
University of California, San Diego

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