IUBio

Cutting problem with SalI

Yigang Tong tongyigang at ROCKETMAIL.COM
Tue May 12 04:28:10 EST 1998


Hi there,

When I cut a plasmid with SalI and SpeI respectively,
SalI can not cut the the plasmid efficiently, while
SpeI does. Sequence analysis showed that the plasmid
contains two SalI sites. The problem should not be
caused by SalI's activity, since the same enzyme can
cut another plasmid efficiently.

Does anyone have such experience with SalI or other
restraint enzyme? Thanks.





 
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