Ger Zandbergen Zandbergen at rullf2.medfac.leidenuniv.nl
Wed May 13 11:08:51 EST 1998


I am interested in specific protocols about deglycosilation. We have a
soluble recombinant protein with 6 potential N linked sugars and
probably 1 or 2 O linked sugars. I want to have the soluble protein if
possible completely without glycosilation. We have already used
N-glycosidase-F but still ended up with 2 protein bands.

I hope someone can help me,


Ger van Zandbergen
Dept of Nephrology,
LUMC, the Netherlands

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