Stable vs Transient transfection

Gabriel Kremmidiotis gkremmid at mad.adelaide.edu.au
Wed May 13 22:31:16 EST 1998

I have been studying the intracellular localization of a gene using GFP
protein chimeras. In transient transfectants (HeLa cells) I observe
cytoplasmic vesicular localization.  In stable HeLa transfectants the
protein localises in the Golgi.  Which of the two is correct ?

Can anyone please help me on this ?

Thank you

Dr Gabriel Kremmidiotis              E-mail:gkremmid at mad.adelaide.edu.au
Centre for Medical Genetics
Women's and Children's Hospital      Telephone
72 King William Road                     (National):     (08)8204 6270
North Adelaide                           (International):(61-8)8204 7342
SA 5006
Australia                            Fax:  8204 7342

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