Stable vs Transient transfection

mark mark.haynes at mail.tju.edu
Fri May 15 15:35:30 EST 1998

Gabriel Kremmidiotis wrote:
> I have been studying the intracellular localization of a gene using GFP
> protein chimeras. In transient transfectants (HeLa cells) I observe
> cytoplasmic vesicular localization.  In stable HeLa transfectants the
> protein localises in the Golgi.  Which of the two is correct ?
> Can anyone please help me on this ?
> Thank you
> Gabriel

It would be hard to say from that description but my guess is both are 
correct and the problem now is to discern how the two locales are 

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