IUBio

Stable vs Transient transfection

mark mark.haynes at mail.tju.edu
Fri May 15 15:35:30 EST 1998


Gabriel Kremmidiotis wrote:
> 
> I have been studying the intracellular localization of a gene using GFP
> protein chimeras. In transient transfectants (HeLa cells) I observe
> cytoplasmic vesicular localization.  In stable HeLa transfectants the
> protein localises in the Golgi.  Which of the two is correct ?
> 
> Can anyone please help me on this ?
> 
> Thank you
> Gabriel


It would be hard to say from that description but my guess is both are 
correct and the problem now is to discern how the two locales are 
related.
markH



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