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> From: Klemens Vierlinger
>> Subject: iga elisa
>> Date: Wed, 27 May 1998 14:44:52 +0200
>>> hi!
> we are trying to establish an elisa to measure the iga level in
> intestinal wahings of mice. but unfortunately we measure levels 50 times
> lower than expected.
> so we suppose that our detection antibody (specific for kappa light
> chain) might be wrong.
> does anyone know something about iga - elisa and about the kappa-lambda
> ratio in the intestine of mice?
> thanks
> klemens
>
Why don't you use biotin labelled alpha-chain specific antibody instead.
And overnight incubation with the washings might help as well.
By the way, are you detecting total iga or specific iga?
Good luck!
<i>Kefei Hu</i>
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<HTML>
<BLOCKQUOTE TYPE=CITE>
<PRE>From: Klemens Vierlinger <klemens.vierlinger at akh-wien.ac.at>
Subject: iga elisa
Date: Wed, 27 May 1998 14:44:52 +0200
hi!
we are trying to establish an elisa to measure the iga level in
intestinal wahings of mice. but unfortunately we measure levels 50 times
lower than expected.
so we suppose that our detection antibody (specific for kappa light
chain) might be wrong.
does anyone know something about iga - elisa and about the kappa-lambda
ratio in the intestine of mice?
thanks
klemens</PRE>
</BLOCKQUOTE>
<BR>Why don't you use biotin labelled alpha-chain specific antibody instead.
And overnight incubation with the washings might help as well.
<P>By the way, are you detecting total iga or specific iga?
<P>Good luck!
<P><i>Kefei Hu</i></HTML>
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