enolase as a substrate

Aki Manninen ltakma at news.cc.tut.fi
Tue Nov 10 02:57:47 EST 1998


Hi everybody!

I'm am looking for a good protocol for in vitro kinase assay (for
Src-family tyrosine kinases) using an exogenous substrate (enolase is one
that I've heard is used). One problem is that enolase is pretty
close to these kinases in molecular weight. I'd appreciate any advice
concerning this assay (conditions, how to treat enolase prior to use, are 
there better substrates etc...

Regards,

-Aki Manninen-




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