enolase as a substrate

John Richard Seavitt jrseavit at artsci.wustl.edu
Wed Nov 11 12:41:44 EST 1998


On Tue, 10 Nov 1998, Aki Manninen wrote:

> I'm am looking for a good protocol for in vitro kinase assay (for
> Src-family tyrosine kinases) using an exogenous substrate enolase ...

Take a look at http://artsci.wustl.edu/~jrseavit/kinase.shtml

Although the size of enolase is 'close' to that of some src-family
members, we run them out on a large PAGE gel apparatus (about 10-12 cm of
resolving gel) and get very good separation.

John




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