mark mark.haynes at
Fri Nov 13 14:39:41 EST 1998

prasad turaga wrote:
> I am testing IL-10 in the culture supernatants of PBMCs.  After
> analysing once, the sups were stored at -70C.  They were again thawed
> and tested again and I did not see any quantities of IL-10 in the
> supernatants.  Is IL-10 in the culture supernants sensitive to freeze
> thawing?


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