cDNA freezing

mark mark.haynes at mail.tju.edu
Fri Nov 13 14:34:04 EST 1998


Glen Ulett wrote:
> 
> Does anyone know how many repeat cycles of freeze-thawing cDNA can
> undergo before significant loss of yield. It is to be used for PCR with
> products up to 500bp. Is it true that even many cycles of freeze-thawing
> would not decrease yield.?
> Glen.ulett at jcu.edu.au



That is my understanding but I wouldn't say ad infinitum--its not true 
with rna though and the best thing to do is to convert the rna to cDNA 
before freezing.



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