Problem with ELISAs
sbe at biochem.usyd.edu.au
Sun Nov 15 17:14:05 EST 1998
How fresh is your H2O2?
We have sometimes seen similar symptoms to yours if the bottle is a bit elderly.
In article <72ht2h$53i$1 at nntp.Stanford.EDU>, robinb at leland.Stanford.EDU
(Robin Blumenthal) wrote:
|| We are having an interesting problem in our lab with our ELISAs. I was
|| wondering if anyone out there might have any ideas.
|| We are doing sandwich ELISAs for cytokines and Igs (both human and murine)
|| using a biotin-linked secondary Ab, strep-HRP and OPD as a substrate. The
|| problem we are having is that the developing has slowed down incredibly.
|| Assays that once took 5 min to develop are now taking over 30 min and
|| still not coming all the way up. We are in the process of testing all the
|| components but in a latest test two different batches of HRP-strep gave
|| the same results. We are a bit stumped.....anyone have any ideas?
|| We would appreciate any ideas!
|| Thank you
|| Robin Blumenthal, Ph.D.
|| Department of Pediatrics, Rm S303
|| Stanford University
|| 300 Pasteur Drive
|| Stanford, California 94305
|| robinb at leland.stanford.edu
|| (650) 723-8224 (650) 498-4632 FAX
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