TNF production

uawl at liverpool.ac.uk uawl at liverpool.ac.uk
Sat Nov 21 11:16:42 EST 1998


I am working with mononuclear cells stimulated with LPS to produce
cytokines. Does anybody know the molecular weight of TNF released in the
supernatant? When I run an SDS-PAGE(reduced) I don't seem to get the
17kd
I know TNF is not a monomer, does that acount for it? If so what are the
molecular weighs of TNF produced by this cells?
I will appreciate any information.

Wuleta Lemma
uawl at liverpool.ac.uk



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