hybridomas

Jay Mone' jmone at MARAUDER.MILLERSV.EDU
Tue Nov 24 09:36:27 EST 1998


A couple of suggestions:

(1)  Increase the serum concentration to 10%.  Be sure you are using 
high-quality, hybridoma-tested FBS.

(2)  After thawing, check viability using trypan blue exclusion.

(3)  Consider growing the cells in smaller flasks (T-25 or even multi 
well plates.  also consider growing the hybridomas over a feeder layer 
of resident peritoneal macrophages or mouse fibroblasts.  This will 
also increase the amount of Ab secreted.

Good luck!

Jay Mone'



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