fibroblasts on beads
k.lehnert at auckland.ac.nz
Wed Nov 25 15:10:01 EST 1998
Sure, many cell types will grow on beads. But will the beads fit through
Check first, as the beads are much bigger than normal cells!
Stephen Knight <stigh at med.unc.edu> schrieb im Beitrag
<36557DAB.68DF at med.unc.edu>...
> Daniel Lottaz wrote:
> > I intend to culture fibroblasts on beads prior to FACS-Analysis. Anyone
> > experience in such a technique?
> > Many thanks for any contribution.
> > Daniel Lottaz, Berne
> > e-mail: daniel.lottaz at zmk.unibe.ch
> Fibroblasts should not be difficult to culture on microcarrier
> beads. I routinely use Pharmacia Cytodex Type III (collagen type 1
> coated) mc-beads to culture McCoy cells for subsequent infection with
> Chlamydia trachomtis. There are two texts that come to mind for general
> information, the first being R. Ian Freshney "Culture of Animal Cells"
> Wiley Liss ISBN 0-471-58966-7, the second being a technical manual
> "Microcarrier cell culture" published by Pharmacia.
> I have also cultured WI-38 and primary human uterine stroma on
> the Cytodex -3 beads with very good results.
> There is a certain amount of fine tuning required for obtaining
> the best culture conditions but it's pretty straight-forward. One
> pitfall is that there because there is usually a higher density of cells
> for the volume of medium in the flask, the pH drop and rate of medium
> depletion is faster than stationary cultures.
> I hope this helps and the mc bead approach works for you.
> Stephen Knight
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