cDNA freezing

Alec Redwood aredwood at cyllene.uwa.edu.au
Wed Nov 25 20:15:58 EST 1998


couldn't give you a number of freeze/thaws you should aim for but it (from my
experience) will certainly decrease your activity, I don't know why as DNA is
usually pretty stable.

mark wrote:

> Glen Ulett wrote:
> >
> > Does anyone know how many repeat cycles of freeze-thawing cDNA can
> > undergo before significant loss of yield. It is to be used for PCR with
> > products up to 500bp. Is it true that even many cycles of freeze-thawing
> > would not decrease yield.?
> > Glen.ulett at jcu.edu.au
>
> That is my understanding but I wouldn't say ad infinitum--its not true
> with rna though and the best thing to do is to convert the rna to cDNA
> before freezing.




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