Lipid and T-cell viability

Derek W.R. Gray derek.gray at nds.ox.ac.uk
Fri Oct 9 11:29:44 EST 1998


If you mean you when yous say 3000 tr/min you are using 3000 Revolutions
Per minute (RPM) in a reasonably large centrifuge the G force is very
high.  If your cells are just in saline they may be being crushed to
death.  12,000 RPM for 3 minutes in a centrifuge with 12 cm radius is
easily sufficient. Also saline is not the best medium for preserving
cell viability-use a recognized medium for lymphocytes such as RPMI 1640
with 10% Fetal calf serum.



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