urgent problem wit AB to proteinG coupling

Oliver Politz politz at medizin.fu-berlin.de
Tue Oct 13 06:59:34 EST 1998


Dear netters,
I just made a big mistake in the attempt to couple IgG to protein G sepahrose and covalently link it with DMP.
The protocol tells that the DMP reaction should be stopped with 50 mM ethanolamine pH 8.2. But what I took was pure ethanolamine (pH 8.2) for a 2 min. and intensive washig with PBS.
 What can I expect ? Is it worth to use this matrix?
Thanks in advance
Oliver
politz at gmx.de




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