DNA-protein interaction
mark
mark.haynes at mail.tju.edu
Thu Oct 22 12:27:51 EST 1998
wan20 at usa.net wrote:
>
> Hi, Does any one know how to determine which nucloetides in a region bind to a
> specific regulator if the sequence of the DNA is known?
>
> Thanks.
>
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Are youasking about EMSA assays? IF so I remember reading a laurie glimcher
paper that dealt with transcription factors for murine IL-4 (nk-at and
ap-1) and they worked out where the factor bound using a panel of oligos that
contained small nucleotide changes. Try immunity 2:473, may 1995
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