DNA-protein interaction

mark mark.haynes at mail.tju.edu
Thu Oct 22 12:27:51 EST 1998


wan20 at usa.net wrote:
> 
> Hi, Does any one know how to determine which nucloetides in a region bind to a
> specific regulator if the sequence of the DNA is known?
> 
> Thanks.
> 
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Are youasking about EMSA assays?  IF so I remember reading a laurie glimcher 
paper that dealt with transcription factors for murine IL-4 (nk-at and 
ap-1) and they worked out where the factor bound using a panel of oligos that 
contained small nucleotide changes. Try immunity 2:473, may 1995



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