Fixation for FITC-dextran

Gordon MacPherson gordon.macpherson at pathology.oxford.ac.uk
Fri Sep 11 09:04:10 EST 1998


Dear all,
    We are trying to label cells for immunofluorescence that have taken
up FITC-dextran by endocytosis .  The problem is that any aqueous medium
leaches out the FITC-dextran.  Is anyone aware of a fixative (perhaps a
cross-linker?) or fixation protocol that will prevent this loss?

Gordon MacPherson




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