Non-autoflourescing nuclear counterstain

[meledy]

Hi all,

I'm on a hunt for a nuclear counterstain to use in an experiment i'm
int he middle of . The problem is that I'm already staining with red
green and blue flourescent secondary antibodies and therefore can't
use any stain that autoflouresces.

Any suggestions???

Also, on a related note, does anyone have any suggestions on
optomizing evans blue staining? I can leave the stuff on (.25% soln)
for 15 minutes and still not see a decent signal.

Thanks

Elizabeth