Check the Current Protocols in Immunology.
You will find lots of good stuff there.
> Dear Sir,
> I'm a graduated student at Dept. of chemistry, Seoul National
> I'm studying an enzyme possibly involved in rat thymus apoptosis.
> I suppose the enzyme activity resides in thymus macrophage.
> If anybody have a protocol for the isolation of macrophage from the
> thymus (I need at least million cells for the activity assay), please
> tell me.
> Thank you in advance.
>> Youngkyun Lee
> Laboratory of enzyme and neurochemistry
> Dept. of chemistry
> Seoul National University
> Seoul, Korea