Hi, there,
I have been using ACK lysing buffer to eliminate chicken RBC from
lymphocytes isolated from spleens. Add 5 ml of ACK buffer and incubated for 5
min at room temp. Centrifuge, wash, and so on....
It works excellent for me.
The formula is in the Current Protocols in Immunology. I did not
remember the formula in detail.
Hua-Chen
HSJ wrote:
> Dear Sirs,
> Someboy knows a manner to eliminate chicken red blood cells of PBMCs. I've
> already looked for this information on the internet and I didn't find
> nothing. If one of you could help me....
> Thanks in advance,
> Hamilton.
