Help needed with s-IgA ELISA

Matthew Bristow mb1 at soc.unl.ac.uk
Thu Sep 24 00:44:42 EST 1998


Hi,

I'm trying to optimise a sIgA ELISA and I have a query that is most likely
blindingly obvious.  I have read that there can be reaction between the
bovine serum albumin  (used to reduce non-specific binding) and the Ig that
your are trying to measure.  Given that s-IgA is fairly 'sticky' Ig, I'm
concerned that s-IgA  in some individuals will bind to BSA and render the
assay less accurate.  Can anybody confirm or deny that this happens and if
it does happen can I limit the problem?

Thanks,

Matt Bristow

University of North London.
School of Health and Sports Science.





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