Matthew Bristow wrote in message <6uduu1$fi7$1 at canard.ulcc.ac.uk>...
>>I'm trying to optimise a sIgA ELISA and I have a query that is most likely
>blindingly obvious. I have read that there can be reaction between the
>bovine serum albumin (used to reduce non-specific binding) and the Ig that
>your are trying to measure. Given that s-IgA is fairly 'sticky' Ig, I'm
>concerned that s-IgA in some individuals will bind to BSA and render the
>assay less accurate. Can anybody confirm or deny that this happens and if
>it does happen can I limit the problem?
>>University of North London.
>School of Health and Sports Science.
There are better blocking agents than BSA - look back ten years or so for
relevant papers, but from memory, fish skin gelatin or casein (dried milk
from a supermarket will do) are both worth trying.