A couple of thoughts:
1. Is it possible that the level of protein epression is now equivilent to
the level of antibody expression. Thus, all the antibody is cleared as
ag/ab complex.
Or, 2. how can you be sure that there is still expression of the viral
antigens. Have you continued to immunize the mice with the DNA vector?
[Curious to hear from others as we are initiating a DNA immunization
program here.]
Regards,
JPC
vinsin at my-dejanews.com wrote in article
<6ut566$4il$1 at nnrp1.dejanews.com>...
>>>> Dear Friends,
>> I have been using DNA vaccine against a viral infection in a murine
model.
> Initially I got good humoral response which is now not being generated.
>> Suggestions invited.
>> Thank you in advance.
>> Vinay
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