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We would like to perform a non-specific esterase stain using alpha-naphtyl =
acetate. We would like to do this in a plastic culture bottle in which =
adherent macrophages/monocytes are cultured. The problem is the fixation =
step. A lot of kits use aceton in their procedures, we believe this could =
react with the plastic.=20
Is there somebody out there that already did a non-specific esterase =
staining in culture bottles, and what fixation procedures did you use?
Or is there a way to collect the monocytes,/macrophages without loosing =
their esterase positivity?=20
Thanks,
Dirk Jochmans
dirk.jochmans at tibotec.be=
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