IUBio

lymphocyte testing revisited

David L. Haviland, PhD dhavilan at IMM2.IMM.UTH.TMC.EDU
Sat Apr 3 14:16:01 EST 1999


On Sat, 3 Apr 1999, Kevin Ault wrote:

> 	I was very interested in the recent post about the Promega
> kits for lymphocyte proliferation testing.  I asked a similar question
> 2-3 years ago to this group.  I got 6-8 replies all stating that
> radiolabeled thymidine was still the "gold standard."  Has this
> changed in the past few years?  Has anyone had trouble submitting to
> peer reviewed journals with these kits?   -  Kevin Ault MD  University
> of Iowa (kevin-ault at uiowa.edu) 

To "second" and extend Ken's question... what of the non-rad cytoxicity
assays?  All I still see in J-Immuno are 51Cr release assays but I was
wondering if anyone has non-rad system operating well?

Thanks in advance,
David

==========================
David L. Haviland, PhD
Assistant Professor, Immunology
University of Texas, HSC - Houston
Institute of Molecular Medicine
2121 W. Holcombe Blvd.
Houston, TX  77030
http://www.uth.tmc.edu/~dhavilan
713.500.2413-Voice
713.500.2424-FAX
==========================







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