Christian Bleiholder wrote:
>> Does anybody know where to get information on
> - reproductive capacity of hiv escape mutants
1)
search MEDLINE at:
http://www3.ncbi.nlm.nih.gov/htbin-post/Entrez/query?db=m&form=0
For example, I tried [immunodeficiency AND "drug resistance" AND
fitness] and got these:
AIDS Res Hum Retroviruses 1998 Aug 10;14(12):1059-64
Impaired fitness of foscarnet-resistant strains of human
immunodeficiency virus type 1.
Tachedjian G, Mellors JW, Bazmi H, Mills J
J Virol 1998 Sep;72(9):7632-7
Resistance-associated loss of viral fitness in human
immunodeficiency virus type 1: phenotypic analysis of
protease and gag coevolution in protease inhibitor-treated
patients.
Mammano F, Petit C, Clavel F
J Infect Dis 1998 May;177(5):1207-13
Altered drug sensitivity, fitness, and evolution of
human immunodeficiency virus type 1
with pol gene mutations conferring multi-dideoxynucleoside
resistance.
Maeda Y, Venzon DJ, Mitsuya H
...
and many more similar citations
2) Look up mutaions in the HIV genome which confer
resistance to anti-HIV drugs here:
http://204.121.6.64:581/Resist_DB/default.htm
This database also contains references to the
primary literature, which may or may not tell the
fitness estimate for each mutation.
> - genomic variation of hiv (especially in the v3 loop) and
> reproductive capacity
See these pages at the HIV sequence database:
http://hiv-web.lanl.gov/http://hiv-web/HTML/97compendium.html
look in part III:
Global Variation in the HIV-1 V3 Region: Priya K. Dighe, Bette Korber
and Brian T. Foley
This is a PDF file for Adobe Acrobat
As far as I know, the V3 loop is not correlated with
reproductive capacity overall. The V3 loop will affect
how well the virus replicates in different cell lines:
The SI, T-cell tropic, CXCR4-using strains have often been found
to be "rapid/high" growers in stimulated T-cell cultures
while the NSI, Macrophage tropic, CCR5-using strains
are often described as "slow-low" growers on stimulated T-cells.
However, each type grows well in cells containing the
appropriate coreceptors (CCR5 or CXCR4).
High positive charge in the V3 loop, particularly at
postions 11 and 25 (counting Cys at the NH2 end of the
Cys-to-Cys V3 loop as position 1), is correllated with
use of the CXCR4 receptor and SI phenotype.
Subtype C rarely becomes charged and is rarely found
to be SI. Subtype D isolates very often have high
positive charge. Other subtypes of HIV-1 are intermediate
between these two extremes.
--
____________________________________________________________________
|Brian T. Foley btf at t10.lanl.gov |
|HIV Database (505) 665-1970 |
|Los Alamos National Lab http://hiv-web.lanl.gov/index.html |
|Los Alamos, NM 87544 U.S.A. |
|____________________________________________________________________|
